Sequestration of the plant secondary metabolite, colchicine, by the noctuid moth Polytela gloriosae (Fab.)

Sajitha, TP and Siva, R and Manjunath, BL and Rajani, P and Navdeep, Gogna and Kavita, Dorai and Ravikanth, G and Uma Shaanker, R (2019) Sequestration of the plant secondary metabolite, colchicine, by the noctuid moth Polytela gloriosae (Fab.). Chemoecology. pp. 1-8.

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Abstract

Colchicine, a well-known alkaloid, is a potent inhibitor of polymerization of tubulin leading to mitotic arrest. It is highly toxic to eukaryotic cells but also widely used in the field of medicine and plant breeding. Gloriosa superba (family: Colchicaceae) is an important natural source of colchicine. The seeds, tubers and leaves of this plant contain about 0.8, 1.2 and 0.014% colchicine by dry weight respectively. A noctuid moth, Polytela gloriosae (family: Noctuidae), feeds voraciously on leaves of G. superba without any adverse effect. However, the fate of colchicine and the mechanisms by which the insect is able to overcome the toxicity of the metabolite is not known. Here, we trace the fate of colchicine in both, the larva and moth of P. gloriosae. Colchicine was quantified in different body parts of the larvae and moth by high performance liquid chromatography, liquid chromatography mass spectrophotometry and nuclear magnetic resonance methods. Of the total colchicine taken in by the larva, a larger portion was excreted, while the rest was sequestered in its cuticle. In the moths however, the wings, legs and antennae were found to accumulate high amount of colchicine. The sequestered colchicine, in both the larva and adult, were chemically identical to that found in the plant. Negligible amounts of demethyl-(−)-colchicine, a less toxic derivative of colchicine was also detected. We discuss the probable adaptive significance of sequestration of colchicine by the insect.

Item Type: Article
Additional Information: Copyright of this article belongs to Springer Nature Switzerland AG 2019
Uncontrolled Keywords: Colchicine · Gloriosa superba · Polytela gloriosae · Sequestration
Subjects: C Publications by ATREEians > G Journal Papers
Divisions: Publications by ATREEians > Journal Papers
Depositing User: The Library Officer
Date Deposited: 12 Jun 2019 06:51
Last Modified: 12 Jun 2019 06:51
URI: http://eprints.atree.org/id/eprint/629

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