Desorption Electrospray Ionization (DESI) Mass Spectrometric Imaging of the Distribution of Rohitukine in the Seedling of Dysoxylum binectariferum Hook. F

Mohana Kumar, P and Srimany, Amitava and Arunan, S and Ravikanth, G and Uma Shaanker, R and Pradeep, T (2016) Desorption Electrospray Ionization (DESI) Mass Spectrometric Imaging of the Distribution of Rohitukine in the Seedling of Dysoxylum binectariferum Hook. F. PLoS ONE, 11 (6). e0158099. ISSN 1932-6203

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Abstract

Ambient ionization mass spectrometric imaging of all parts of the seedling of Dysoxylum binectariferum Hook. f (Meliaceae) was performed to reconstruct the molecular distribution of rohitukine (Rh) and related compounds. The species accumulates Rh, a prominent chromone alkaloid, in its seeds, fruits, and stem bark. Rh possesses anti-inflammatory, anticancer, and immuno-modulatory properties. Desorption electrospray ionization mass spectrometry imaging (DESI MSI) and electrospray ionization (ESI) tandem mass spectrometry (MS/MS) analysis detected Rh as well as its glycosylated, acetylated, oxidized, and methoxylated analogues. Rh was predominantly distributed in the main roots, collar region of the stem, and young leaves. In the stem and roots, Rh was primarily restricted to the cortex region. The identities of the metabolites were assigned based on both the fragmentation patterns and exact mass analyses. We discuss these results, with specific reference to the possible pathways of Rh biosynthesis and translocation during seedling development in D. binectariferum.

Item Type: Article
Additional Information: Copyright of this article belongs to the Authors
Subjects: C Publications by ATREEians > G Journal Papers
Divisions: Publications by ATREEians > Journal Papers
Depositing User: The Library Officer
Date Deposited: 21 May 2019 09:42
Last Modified: 21 May 2019 09:42
URI: http://eprints.atree.org/id/eprint/626

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