Isolation of endophytic bacteria producing the anti-cancer alkaloid camptothecine from Miquelia dentata Bedd. (Icacinaceae)

Singh, Shweta and Hima Bindu, J and Raghu, J and Suma, HK and Manjunatha, BL and Mohana Kumar, P and Ravikanth, G and Nataraja, KN and Ganeshaiah, KN and Uma Shaanker, R (2013) Isolation of endophytic bacteria producing the anti-cancer alkaloid camptothecine from Miquelia dentata Bedd. (Icacinaceae). Phytomedicine, 20 (10). pp. 913-917. ISSN 0944-7113

[img] Text
Phy_ravikanth_vol_20_no_10_2013.pdf - Published Version
Restricted to Registered users only

Download (847kB) | Request a copy

Abstract

Camptothecine (CPT), a quinoline alkaloid, is a potent inhibitor of eukaryotic topoisomerase I. Because of this activity, several semi-synthetic derivatives of CPT are in clinical use against ovarian and small lung cancers. Together with its derivatives, CPT is the third largest anti-cancer drug in the world market. CPT is produced by several plant species belonging to the Asterid clade. In the recent past, several studies have reported the production of CPT by endophytic fungal associates of some of these plant species. In this paper, we report the production of CPT by endophytic bacteria isolated from Miquelia dentata Bedd. (Icacinaceae). Besides CPT, the bacteria also produced 9-methoxy CPT (9-MeO-CPT), in culture, independent of the host tissue. The chemical nature of CPT and 9-MeO-CPT was determined by LC–MS and ESI-MS/MS analysis, and was shown to be similar to that produced by the host tissue. One of the bacterial isolates examined, showed indications of attenuation of CPT production through sub-culture. This is the first report of production of CPT by endophytic bacteria. The identity of the bacteria was ascertained by Gram staining and 16s rRNA sequencing. We discuss the possible mechanisms that might be involved in the synthesis of CPT by endophytic bacteria.

Item Type: Article
Additional Information: Copyright of this article belongs to the Elsevier
Uncontrolled Keywords: Miquelia dentata Western Ghats Endophytic bacteria Camptothecine Alkaloid Anti-cancer
Subjects: C Publications by ATREEians > G Journal Papers
Divisions: Publications by ATREEians > Journal Papers
Depositing User: The Library Officer
Date Deposited: 21 May 2019 09:25
Last Modified: 21 May 2019 09:25
URI: http://eprints.atree.org/id/eprint/624

Actions (login required)

View Item View Item