Authentication of Garcinia fruits and food supplements using DNA barcoding and NMR spectroscopy

Seethapathy, GS and Tadesse, Margey and Santhosh Kumar, JU and Gunaga, Srikanth V and Vasudeva, R and Malterud, Karl Egil and Uma Shaanker, R and de Boer, Hugo J and Ravikanth, G and Wangensteen, Helle (2018) Authentication of Garcinia fruits and food supplements using DNA barcoding and NMR spectroscopy. Scientific Report, 10561.

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Abstract

Garcinia L. (Clusiaceae) fruits are a rich source of (−)-hydroxycitric acid, and this has gained considerable attention as an anti-obesity agent and a popular weight loss food supplement. In this study, we assessed adulteration of morphologically similar samples of Garcinia using DNA barcoding, and used NMR to quantify the content of (−)-hydroxycitric acid and (−)-hydroxycitric acid lactone in raw herbal drugs and Garcinia food supplements. DNA barcoding revealed that mostly G. gummi-gutta (previously known as G. cambogia) and G. indica were traded in Indian herbal markets, and there was no adulteration. The content of (−)-hydroxycitric acid and (−)-hydroxycitric acid lactone in the two species varied from 1.7% to 16.3%, and 3.5% to 20.7% respectively. Analysis of ten Garcinia food supplements revealed a large variation in the content of (−)-hydroxycitric acid, from 29 mg (4.6%) to 289 mg (50.6%) content per capsule or tablet. Only one product contained quantifiable amounts of (−)-hydroxycitric acid lactone. Furthermore the study demonstrates that DNA barcoding and NMR could be effectively used as a regulatory tool to authenticate Garcinia fruit rinds and food supplements.

Item Type: Article
Additional Information: Copyright of this article belongs to the Authors
Subjects: C Publications by ATREEians > G Journal Papers
Divisions: Publications by ATREEians > Journal Papers
Depositing User: The Library Officer
Date Deposited: 21 May 2019 09:11
Last Modified: 21 May 2019 09:11
URI: http://eprints.atree.org/id/eprint/622

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